Theatre Layout and Design
- ICU, A&E, Surgical Wards, Radiology, Pathology
Zones
- Outer Zone: Reception
- Clean Zone: Reception up to the theatre itself
- Aseptic Zone: Theatres
- Disposal Zone: Dirty corridor for disposal
Note: Zones should be linked via corridors that prevent cross-contamination.
Within Theatre
Control of Key Factors
- Temperature and Humidity:
- Controlled via ventilation and thermostats.
- Patients under anaesthesia are at risk of hypothermia.
- Optimal theatre temperature: 25°C to prevent hypothermia; 19°C for surgical temperature.
- Solutions: Bair Hugger, warmed fluids, blankets, airflow mattress.
- Higher temperature needed for paediatric patients due to larger body surface area.
- Optimal Humidity: Around 50%.
- Light:
- Illumination at the wound site should be 40,000 lux.
- Satellite lights: Positioned directly, although they generate heat and convection currents.
Bacterial Contamination in Theatre
Sources of Infection
- Patient
- Instruments
- Surgeon
- Airborne (95% of infections)
Airborne Contamination
- Primarily due to people in the theatre (talking, shedding microorganisms, skin, and clothing).
- 90% of airborne bacteria come from below neck level, especially from axillae and groins.
Measuring Bacterial Contamination of the Air
- Measured in Colony Forming Units (CFU) or Bacteria Carrying Particles (BCP) per cubic meter.
- Microbiological Volumetric Slit Sampler: Draws air over a culture plate, incubated for 48 hours, then counts colonies formed.
Frequency of Measurement
- Plenum theatres: Every time they are refurbished.
- Extra clean theatres: Every 3 months.
Acceptable CFU/m3 values
- Plenum: <35 CFU & <1 CFU of Clostridium Perfringens and Staphylococcus Aureus.
- Ultra-Clean: <20 CFU in the periphery and <10 CFU in the centre.
Ventilation
Key Points
- The ideal air system should:
- Provide a bacteria-free source of air.
- Use positive pressure to displace contaminated air from the operative field.
How Air Enters the Theatre
- Air enters via roof-level HEPA filters (removing particles of 0.5 microns with 99.9% efficiency).
- Filters must be monitored regularly.
Ventilation Systems
| Type of Flow |
Description |
Pros |
Cons |
| Plenum |
Positive pressure maintained by air entering via ceiling/wall diffusers and exiting via floor vents. Air changed 20x per hour. |
Reduces ingress of contaminated air |
Turbulence due to personnel movement, door openings. Each door opening introduces 2m3 of contaminated air. |
| Laminar Flow |
Uniform velocity, unidirectional air flow, usually vertical. |
Shown to reduce infection in some surgeries |
Equipment and personnel can deflect contaminated air towards the wound. |
Types of Laminar Flow
- Horizontal Laminar Flow:
- HEPA filters on walls; airflow across the entire theatre.
- Reduces infection in total hip replacement but increases infection in total knee replacement.
- Vertical Laminar Flow:
- Introduced by Charnley.
- Room within a room concept, air enters through HEPA filters and moves downwards.
- Most commonly used type.
- Problems occur if personnel or equipment deflect airflow.
- Exponential Flow (Ex Flow) System:
- Overcomes vertical flow problems.
- Air flows down and out in a trumpet shape.
- Requires fewer air changes per hour.
Clinical Effects of Laminar Flow
- MRC 1982 RCT: Found vertical laminar flow to be more effective in reducing wound contamination.
- Charnley: Demonstrated a reduction in deep infections with laminar flow.
MRC Trial Findings on Reducing Deep Joint Sepsis
| Method |
Effectiveness |
| Antibiotic Cement |
11-fold reduction |
| Systemic Antibiotics |
5-fold reduction |
| Ultra-clean air |
2.5-fold reduction |
| Body Suits |
2-fold reduction |
Clothing
Ideal Clothing Characteristics
- Prevent shedding of airborne bacteria.
- Effective when wet.
- Allows air and water vapor circulation.
| Material |
Pore Size |
Features |
Disadvantages |
| Standard Cotton |
80 microns |
Comfortable |
Poor bacterial migration prevention, strike-through problem. |
| Ventile |
50 microns |
Reduced pore size |
Uncomfortable, not breathable. |
| Gore Tex |
0.2 microns |
Breathable |
Heavy, expensive. |
| Disposable Non-Woven |
Variable |
Traps bacteria, prevents migration |
Single-use, expensive. |
Body Exhaust Suits
- Maintain negative pressure within the gown, preventing bacteria escape.
Surgical Drapes
- Disposable Non-Woven Drapes: Commonly used to prevent bacterial migration.
- Incision Drapes: No independent benefit shown, primarily used to keep body drapes attached to the skin.
Masks
- Recommended for all personnel in orthopaedic procedures.
- Prevent upper respiratory tract bacterial contamination (5% of airborne bacteria).
- Evidence shows only those near the wound need masks.
Gloves
- Protect both the wound and surgeon.
- Glove Changing:
- Before making an incision (due to contamination during prepping and draping).
- Before handling implants (due to glove colonization during a procedure).
- Double Gloving: 40% incidence of glove perforation in orthopaedic procedures, so double gloving is advised.
Written/reviewed by Kishore Puthezhath
Professor of Orthopaedics and Consultant Paediatric Orthopaedic Surgeon
FRCS (Tr & Orth) revision resource
Reviewed: September 2026
Core revision references: Miller's Review of Orthopaedics; Campbell's Operative Orthopaedics; Orthobullets. Current specialty guidelines are linked within individual notes where applicable.